polyclonal rabbit antitlr4 antibody Search Results


tlr4 polyclonal antibody  (Bioss)
94
Bioss tlr4 polyclonal antibody
Tlr4 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr4 polyclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
tlr4 polyclonal antibody - by Bioz Stars, 2026-02
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tlr4 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation tlr4 antibody
Tlr4 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr4 antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
tlr4 antibody - by Bioz Stars, 2026-02
94/100 stars
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tlr4  (Cusabio)
93
Cusabio tlr4
Figure 3. OxLDL and PA synergize to suppress T cell activity via CD36-mediated innate immune signaling in AML cells (A and B) THP-1 cells (A) or MV4-11 cells (B) stimulated in CM or LDM supplied with different lipids (palmitate [PA], 20 mM; OxLDL, 25 mg/mL) for 3 days were further used for T cell proliferation assay (n = 3). (C–E) Immunoblot analysis of <t>TLR4</t> (C and E) or LYN (D) precipitated proteins. (C and E) AML cells were treated with different reagents (PA, 20 mM; OxLDL, 25 mg/mL; PP1, 10 mM) for 5 h before immunoprecipitation. (F) MV4-11 cells treated with LDM supplied with different reagents (OxLDL, 25 mg/mL; TAK-242, 10 mM; PP1, 10 mM) for 3 days were further used for T cell proliferation assay (n = 3). (G–I) AML cells labeled with a chemical probe (alk-C16) for 4 h in the absence (G and H) or presence of SSO (50 mM) (I) were used for palmitoylation assay.
Tlr4, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr4/product/Cusabio
Average 93 stars, based on 1 article reviews
tlr4 - by Bioz Stars, 2026-02
93/100 stars
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anti-tlr4 rabbit polyclonal antibody d121751  (Sangon Biotech)
90
Sangon Biotech anti-tlr4 rabbit polyclonal antibody d121751
A qPCR showed increased <t>TLR4</t> expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Anti Tlr4 Rabbit Polyclonal Antibody D121751, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-tlr4 rabbit polyclonal antibody d121751/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
anti-tlr4 rabbit polyclonal antibody d121751 - by Bioz Stars, 2026-02
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rabbit polyclonal antitlr4 antibody #gb11186  (Servicebio Inc)
90
Servicebio Inc rabbit polyclonal antitlr4 antibody #gb11186
A qPCR showed increased <t>TLR4</t> expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Rabbit Polyclonal Antitlr4 Antibody #Gb11186, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antitlr4 antibody #gb11186/product/Servicebio Inc
Average 90 stars, based on 1 article reviews
rabbit polyclonal antitlr4 antibody #gb11186 - by Bioz Stars, 2026-02
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anti-tlr4 antibody  (StressMarq)
93
StressMarq anti-tlr4 antibody
A qPCR showed increased <t>TLR4</t> expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Anti Tlr4 Antibody, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-tlr4 antibody/product/StressMarq
Average 93 stars, based on 1 article reviews
anti-tlr4 antibody - by Bioz Stars, 2026-02
93/100 stars
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Image Search Results


Figure 3. OxLDL and PA synergize to suppress T cell activity via CD36-mediated innate immune signaling in AML cells (A and B) THP-1 cells (A) or MV4-11 cells (B) stimulated in CM or LDM supplied with different lipids (palmitate [PA], 20 mM; OxLDL, 25 mg/mL) for 3 days were further used for T cell proliferation assay (n = 3). (C–E) Immunoblot analysis of TLR4 (C and E) or LYN (D) precipitated proteins. (C and E) AML cells were treated with different reagents (PA, 20 mM; OxLDL, 25 mg/mL; PP1, 10 mM) for 5 h before immunoprecipitation. (F) MV4-11 cells treated with LDM supplied with different reagents (OxLDL, 25 mg/mL; TAK-242, 10 mM; PP1, 10 mM) for 3 days were further used for T cell proliferation assay (n = 3). (G–I) AML cells labeled with a chemical probe (alk-C16) for 4 h in the absence (G and H) or presence of SSO (50 mM) (I) were used for palmitoylation assay.

Journal: Cell reports. Medicine

Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML.

doi: 10.1016/j.xcrm.2024.101592

Figure Lengend Snippet: Figure 3. OxLDL and PA synergize to suppress T cell activity via CD36-mediated innate immune signaling in AML cells (A and B) THP-1 cells (A) or MV4-11 cells (B) stimulated in CM or LDM supplied with different lipids (palmitate [PA], 20 mM; OxLDL, 25 mg/mL) for 3 days were further used for T cell proliferation assay (n = 3). (C–E) Immunoblot analysis of TLR4 (C and E) or LYN (D) precipitated proteins. (C and E) AML cells were treated with different reagents (PA, 20 mM; OxLDL, 25 mg/mL; PP1, 10 mM) for 5 h before immunoprecipitation. (F) MV4-11 cells treated with LDM supplied with different reagents (OxLDL, 25 mg/mL; TAK-242, 10 mM; PP1, 10 mM) for 3 days were further used for T cell proliferation assay (n = 3). (G–I) AML cells labeled with a chemical probe (alk-C16) for 4 h in the absence (G and H) or presence of SSO (50 mM) (I) were used for palmitoylation assay.

Article Snippet: After blocking with 5% nonfat milk in TBST, the membranes were incubated with the the following antibodies overnight 4 C with constant rotation: p-p65 (Cell Signaling Technology; 3033S), p65 (Cell Signaling Technology; 8242S), actin (Cell Signaling Technology; 3700S), Lamin-B1 (Santa Cruz Biotechnology; sc-374015), MYD88 (Cell Signaling Technology; 4283), CD36 (abcom; ab133625), ZDHHC6 (abcom; ab121423), TLR4 (CUSABIO Technology; CSB-PA001434), LYN (Cell Signaling Technology; 2796S).

Techniques: Activity Assay, Proliferation Assay, Western Blot, Immunoprecipitation, Labeling

A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Expressing, Binding Assay, Luciferase, Western Blot

A The diagram presented the xenograft modeling process. B Euthanized mouse to display the subcutaneous xenografted tumors. C 216911KO group resulted in much smaller tumors in size compared to the control group. D 216911KO group resulted in much smaller tumors in weight compared to the control group. The histogram represents the mean tumor weight of each group. ***p < 0.001 by student T tests. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway in vivo. Both TLR4 and p-p65 levels were elevated in tumors formed in the 216911KO group compared to the control group.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A The diagram presented the xenograft modeling process. B Euthanized mouse to display the subcutaneous xenografted tumors. C 216911KO group resulted in much smaller tumors in size compared to the control group. D 216911KO group resulted in much smaller tumors in weight compared to the control group. The histogram represents the mean tumor weight of each group. ***p < 0.001 by student T tests. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway in vivo. Both TLR4 and p-p65 levels were elevated in tumors formed in the 216911KO group compared to the control group.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Control, Western Blot, In Vivo

A The diagram presented the clinical HCC tumor tissue sampling process. B The immunohistochemistry demonstrates that TLR4 was downregulated in high pir-hsa-216911 tumor samples. The image shows typical IHC results. The box plot presents all IHC scores. *p < 0.01 by LSD test. C The immunohistochemistry demonstrates that c-GSDMD activation was suppressed in high pir-hsa-216911 tumor samples. The image shows typical IHC results. The box plot presents all IHC scores. * p < 0.01 by LSD test. D The tumor/adjacent tissue pir-hsa-216911 ratio was higher in HCC patients accompanied by steatosis. ** p < 0.01 by student T tests.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A The diagram presented the clinical HCC tumor tissue sampling process. B The immunohistochemistry demonstrates that TLR4 was downregulated in high pir-hsa-216911 tumor samples. The image shows typical IHC results. The box plot presents all IHC scores. *p < 0.01 by LSD test. C The immunohistochemistry demonstrates that c-GSDMD activation was suppressed in high pir-hsa-216911 tumor samples. The image shows typical IHC results. The box plot presents all IHC scores. * p < 0.01 by LSD test. D The tumor/adjacent tissue pir-hsa-216911 ratio was higher in HCC patients accompanied by steatosis. ** p < 0.01 by student T tests.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Sampling, Immunohistochemistry, Activation Assay

Correlation analysis between pir-hsa-216911 elevation, TLR4 expression, and GSDMD activation.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: Correlation analysis between pir-hsa-216911 elevation, TLR4 expression, and GSDMD activation.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Expressing, Activation Assay